Search results for "Attenuated total reflectance"

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ATR-FTIR spectroscopy for the routine quality control of exosome isolations

2021

Abstract Exosomes are nanosized vesicles containing specific cargos of DNA, RNA, proteins, metabolites, and intracellular and membrane lipids. Exosome isolation needs to be optimized carefully depending on the type of biofluid and tissue and the retrieved exosomes need to be characterized. The main objective of this study was to determine the feasibility of a multimodal analysis of Attenuated Total Reflectance – Fourier Transform Infrared (ATR-FTIR) spectroscopy and UPLC–QqTOF-MSMS for the development of a routine quality control tool of isolated exosomes and the rapid characterization of their lipid profiles and total protein content. Using human milk as model example, exosomes were isolat…

Fourier transform infrared (ATR-FTIR)Membrane lipidsOmicsExosomes01 natural sciencesExosomeAnalytical Chemistry03 medical and health sciencesstomatognathic systemMETLINSpectroscopy030304 developmental biology0303 health sciencesChemistryProcess Chemistry and TechnologyVesicleHuman milk010401 analytical chemistryExtracellular vesiclesSphingolipidMicrovesicles0104 chemical sciencesComputer Science ApplicationsBiochemistryAttenuated total reflectionLipidomicslipids (amino acids peptides and proteins)UltracentrifugeAttenuated total reflectanceSoftwareChemometrics and Intelligent Laboratory Systems
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